Part:BBa_K2624004:Experience

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Applications of BBa_K2624004

We used this part to construct the "p-H*-U6" plasmid in order to express the inhibitory strand to inhibit the effect of RNA interference caused by miRNA. The results of qPCR (Real-time Quantitative Polymerase Chain Reaction) shows that the effect of inhibitory strand was obvious, which reduced the expression of miRNA and increase the expression of MAP4K4 mRNA.

Figure. The expression of MAP4K4 miRNA (A) and MAP4K4 mRNA (B)

"p-H*" contains "HULC-pri-miRNA" parts; "p-H*-U6" contains "HULC-pri-miRNA-U6-pSil" parts;

"p-H" contains "HULC--pri-miRNA(MAP4K4)" parts; "Plasmid1" contains "tTA-NS5B-NLS" parts; "Plasmid2" contains "HULC--pri-miRNA(MAP4K4)-U6-pSil-TRE-sponge" parts;

User Reviews

UNIQ8ffd17081fee332d-partinfo-00000000-QINU UNIQ8ffd17081fee332d-partinfo-00000001-QINU